WBBSE 9th Class Science Solutions Life Science & Environment Chapter – 4 Biology and Human Welfare

WBBSE 9th Class Science Solutions Life Science & Environment Chapter – 4 Biology and Human Welfare

West Bengal Board 9th Class Science Solutions Life Science & Environment Chapter – 4 Biology and Human Welfare

WBBSE 9th Class Life Science & Environment Solutions

TOPIC – 1

IMMUNITY AND HUMAN DISEASES

SUMMARY

  • The ability of the host to fight and protect against disease-causing organism conferred by the immune system is called immunity.
  • Immunity is of two types-(i) Innate Immunity (ii) Acquired Immunity.
  • Innate immunity is a non-specific type of immunity in an individual that is present at the time of birth. It works by preventing the entry of pathogens through various barriers. There are various types of barriers such as physical barrier (skin), chemical barrier (lysozyme), by which the body prevents the entry of foreign agents into our body.
  • Acquired immunity is pathogen specific and is characterised by a memory of the activities of a pathogen in the body of a living organism. When the pathogen is encountered for the first time a primary response of low intensity is produced. Subsequent encounters with the same pathogen produces high intensity response of high intensity with the help of memory cells. This is called secondary or anamnestic response.
  • Primary and secondary response are carried out with the help of 2 special types of lymphocytes-B lymphocytes and T lymphocytes. B lymphocytes produce special proteins called antibodies whereas the T lymphocytes help B lymphocytes to produce antibodies.
  • Antigen is a substance usually protein in nature (sometimes polysaccharide or lipid) that generates a specific immune response and induces the formation of specific antibody or specially sensitised T cells or both.
  • There are different types of antigens on the basis of antigen: (i) Exogenous antigens are the foreign particles that enter the body system from outside, start circulating in body fluids and are phagocytosed by the antigen processing cells. (ii) Endogenous antigens are the antigenic products, subfragments or any compounds that are produced by the body itself.
  • An immune response is a reaction which occurs within an organism for the purpose of defending against foreign invaders.
  • Humoral immune response is a kind of immune response that is involved in production of antibodies.These antibodies are present in the blood.
  • Cell-mediated immunity is an immune response that does not involve antibodies, but rather involves the activation of phagocytes, antigen-specific cytotoxic T-lymphocytes, and the release of various cytokines in response to an antigen. The body is able to differentiate between ‘self and ‘non-self’ and the cell-mediated immune response is responsible for the graft rejection.
  • When a host is exposed to antigens, which may be in the form of living or dead microbes or other proteins, antibodies are produced in the host body. This type of immunity is called active immunity. called active Examples-vaccines like BCG, MMR.

Long Answer Type Questions

1. Briefly describe about the different types of immunity.
Ans. Different types of immunity
Immunity is of two types-
  1. Innate or natural or non-specific Immunity: The innate immunity refers to non-specific defence mechanisms that come into play immediately or within hours of an antigen’s appearance in the body.It consists of various types of physical, and physiological barriers. Example-skin (physical barrier) prevents entry of microorganisms; mucous membrane, acid of the stomach (physiological barrier) kills most microorganisms.
  2. Acquired immunity or adaptive or specific Immunity: The immunity that an individual acquires after birth is called acquired immunity. It is specific and mediated by antibodies or lymphocytes or both which make the antigen harmless. It is of two types, active immunity and passive immunity.
    1. Active Immunity: In this case, person’s own cells produce antibodies in response to infection. or vaccination. It is slow and takes time in the formation of antibodies. It is long lasting and is harmless. Active immunity may be natural or artificial (1) A person who has recovered from an attack of small pox or measles or mumps develops natural active immunity. (ii) Artificial active immunity is the resistance induced by vaccines. Examples of vaccines are-BCG vaccine, MMR vaccine, etc.
    2. Passive Immunity: When ready-made antibodies are directly injected in an individual against foreign agents, it is called passive immunity. It provides immediate relief but is not long lasting. It may be natural or artificial-(i) Natural passive immunity is the resistance passively transferred from mother to the foetus through placenta or through colostrum (a yellowish fluid secreted initial days of lactation). (ii) Artificial passive immunity is the resistance passively transferred to a recipient by administration of antibodies. For example, Anti tetanus serum (ATS) is prepared in horses by active immunisation of horses with tetanus toxoid, withdrawal of blood and separation of the serum. ATS is used for passive immunisation against tetanus.
2. Write the characteristics or features of antigen. How you recognise antigen?
Or, State the properties of antigen.
Ans. Characteristics/features of antigen
The features or characteristics of antigen are as follows-
  1. Foreignness: The immune system normally discriminates between self and non-self such that only foreign molecules are immunogenic. When an antigen is introduced into an organism, the degree of its immunogenicity depends on the degree of its foreignness.
  2. Molecular size: In general, the larger the molecule the more immunogenic it is likely to be. The best immunogens tend to have a molecular mass approaching 100,000 Da. The immunogens with lower efficiency generally have a molecular size between 5000-10000 Da.
  3. Chemical composition: They are structurally protein in nature. Sometimes it may be composed of polysaccharide, lipid and nucleic acid.
  4. Antigen binding site: It has specific region that interacts with antibody (Ab) which is known as epitope.
Properties of antigen
Antigens can be recognised by some of its properties. The properties of antigens are-
  1. Antigenicity: The ability to cause the production of antibodies.
  2. Antigenic specificity: The ability to enter into an interaction with the corresponding antibodies.
  3. Allergenicity: Having the capacity to induce allergy (hypersensitivity).
  4. Immunogenicity: Property inducing a detectable immune response.
3. Mention the characteristic features of antibodies. Briefly describe the structure of the most common antibody present in human plasma. 
Ans. Characteristics features of antibody
Characteristic features of antibodies are as follows-[1] Antibodies are glycoproteins commonly called immunoglobulins. [2] Molecular weight of an antibody ranges from 150000 to 950000 Da. [3] Antibodies are antigen-specific.
Structure of antibody
Immunoglobulin G or IgG is the most common antibody present in human plasma. The structure of IgG is mentioned below. [1] Immunoglobulin G molecule is a ‘Y’-shaped structure. [2] Each molecule has a specific antigen-binding site, by which it attaches with a specific antigen to inactivate it. [3] The immunoglobulin molecule is composed of 4 polypeptide chains of which two are heavier and two others are lighter in weight. These chains remain attached with di-sulphide bonds. [4] The two forked arms are made up of one light and one heavy chain each. The stem arm of IgG molecule is composed of two heavy chains.
4. What is humoral immunity? How does humoral immunity work?
Ans. Humoral immunity
The immune response, which involves the action of different B lymphocytes, like plasma cells, for producing antigen-specific antibodies and memory cells for neutralising or eliminating toxins and pathogens in the blood and lymph is called humoral immunity.
Mechanism of humoral immunity
Humoral immunity works for both primary and secondary immune response. In primary response B lymphocytes develop typical antibody-producing plasma cells. These cells produce antigen specific antibodies. Antibodies work in following four ways.
  1. Agglutination: By this process antibodies agglutinate pathogens into clusters, which are then collectively phagocytised by macrophages.
  2. Precipitation: Some antigen molecules are clamped by antibodies and are precipitated.
  3. Opsonisation: Antibodies form a covering on the antigens so that the phagocytic cells can ingest packed antigens.
  4. Neutralisation: Certain toxic antigens are detoxified and inactivated by the antibodies.
In secondary response memory B cells play the major role. These cells are produced during primary immune response and remain stored in the secondary lymphoid tissues for keeping the immunological behaviour of the pathogen in memory of the body’s immune system. In case of another attack, these cells help in quick activation of body’s immune system.
5. Mention the roles of different T cells in immune response? Mention the roles of different B cells in immune response? 
Ans. Roles of different T cells
Different T cells or T lymphocytes play different roles in immune response of the body. They are as follows-[1] Cytotoxic T cells or TC cells kill the infected cell, to destroy antigen carrying pathogens within it. [2] Helper T cells or TH cells enhance the activity of killer cells (a type of Tc cells), antibody producing B cells and phagocytic macrophages. [3] Suppressor T cells or T cells destroy Tc cells, TH cells and B cells to stop immune response after the elimination of the antigens.
Roles of different B cells
Different B cells or B lymphocytes play different roles in immune response of the body. They are as follows—[1] Plasma cells originated from B cells produce specific antibodies to inactivate antigens. [2] Memory B cells remain in lymph glands, keeping the nature of antigens in memory, take quick action in case of any further attack.
6. Briefly describe the mechanism of immune response against viral attack in human body.
Ans. Mechanism of immune response against a viral attack
Immune response is a complex process involving various cells and biochemical components inside the body. The steps of immune response against viral antigen are as follows-[1] As any virus reaches blood or attacks any body cells, large phagocytic macrophages engulf those infected cells. Soon viral antigens appear on the cell surface of macrophages. [2] High concen-tration of viral antigens on macrophage activates helper T lymphocytes in blood. [3] Helper T the cells immediately initiate production of cytotoxic killer T cells, memory T cells and B lymphocytes. [4] B lymphocytes proliferate very fast to produce plasma cells and memory B cells. [5] Plasma cells produce specific antibodies to bind and inactivate virus before they get chance to infect a cell. [6] Killer T cells destroy the infected body cells along with viruses. [7] T and memory B cells stay in the body to recognise the same pathogen in case of further infection and to develop quicker response.
7. Give a brief historical account of the discovery of small pox vaccine.
Ans. Discovery of small pox vaccine
The scientific concept of vaccination was developed in the later half the of 18th century when a number of scientists and physicians were working hard to develop a vaccine against small pox, the most deadly and contagious disease of that time. Scientists noticed that most individuals, who once suffered from cow pox safely bypassed small pox. But the search was on for long 25 years. Then Dr. Edward Jenner (1798) broke through the concept of vaccination. He collected a little pus from a cow pox vesicle on the hand of Sarah Nelmes, a milkmaid, and introduced it into the arm of James Phipps, an eight year old boy. After two months, the boy was inoculated with small pox virus, but he did not develop the disease. The modern concept of vaccination is standing on this story. After this discovery, the practice of vaccination took the correct route. After a long battle for about two centuries, small pox has now been eradicated from the world.
8. What is a vaccine? Mention the basic working principles of vaccines to develop immunity in the body. 
Ans. Vaccine
Vaccine is an antigenic material that is prepared with killed or weakened pathogens, part of pathogens, bacterial toxins or microbial proteins, which do not cause illness but provide active acquired immunity against those pathogens when introduced into the body.
Working principle of vaccines
By vaccination, a killed germ, attenuated (weakened) germ, toxoids (toxins collected from germs) or subunits (fragments of proteins from germs) are introduced into the body of an individual. These may be injected or administered orally. As soon as the substance enters the body, antigens present in it, activate the immune system and initiate an immune response. T cells and B cells take a little time to inactivate such little quantity of antigens and this process is known as primary immune response. However, in this case, main function is done by memory B cells. These cells, produced during the immune response, are retained in lymph nodes such as spleen, thymus, etc. for a long time. In case of any future infection by the same germ, these memory cells recognise those antigens instantly and inactivate or destroy the attacker very fast. This process is recognised as secondary immune response. Thus, a successful vaccination immunises a person effectively.
9. Give a brief account of different types of vaccines.
Ans. Types of vaccines
With the development of science, new and advanced varieties of vaccines are being developed. Till date World Health Organization (WHO) has licensed vaccines for 25 diseases. Different types of vaccines are-
  1. Killed vaccine: These vaccines contain killed pathogens. Vaccines of cholera, plague, influenza are of this type.
  2. Live attenuated vaccine: By some laboratory techniques virulence of the pathogens are reduced to prepare this type of  vaccines. Vaccines of mumps, measles, and rubella (MMR vaccine) are of this type.
  3. Toxoid vaccine: This type of vaccine is prepared by inactivated toxins from pathogens. Vaccines of tetanus, diphtheria are of this type.
  4. Conjugate vaccine: Generally polysaccharide of bacterial capsule is attached with a protein to prepare this vaccine. Body’s immune system can recognise this conjugated protein and keep it in memory for future immune response. Vaccine against Haemophilus influenzae type-B is of this type.
  5. Sub-unit vaccine: This type of vaccine is prepared by small fragments of pathogenic protein. Vaccine against Hepatitis B is of this type.
  6. Heterotypic vaccine: This is prepared by pathogens, causing disease to other animals but less or non-virulent to human. Vaccines of small pox and tuberculosis are of this kind.
10. Explain the importance of hand washing. Write the components used in washing.
Ans. Importance of hand washing
  1. Reducing number of infections: Hand washing can prevent about 30% of diarrhoea related sickness and about 20% of respiratory infections (e.g. common cold, meningitis, flu, hepatitis A) and most types of infectious diseases.
  2. Reducing child death: About 1.8 million children under the age of 5 die every year from pneumonia and diarrhoea related diseases. Washing hands carefully and thoroughly can help children and child care providers stay healthy.
  3. To remove dirt: Removing dirt through hand washing may even help prevent skin and eye infections.
Components used in washing
  1. Water: Pure distilled water is the main component used in hand washing.
  2. Soap and Detergent: Water is not efficient for cleaning skin because fats and proteins those are components of organic oil are not readily dissolved in water. Removal of micro-organisms from skin may be enhanced with the use of soaps and detergents.
  3. Hand antiseptic: Hand sanitizers containing a minimum of 60% to 70% alcohol are and efficient germ killer.
  4. Shampoo: Shampoos that contain selenium are effective in controlling dandruff. Dandruff is caused owing to fungal infection on the scalp.
  5. Thimerosal: An organic compound containing mercury can be used for dis infection of skin and instruments.
  6. Wipes: Hand washing with wipes is also recommended by CDC (Centers for Disease Control and Prevention). This washing is convenient during travelling when water and soap will not be available.
  7. Toothpaste and mouth wash: It is used in brushing teeth and washing mouth.
  8. Disinfectant: Phenyl, bleaching powder is applied in our surrounding to keep the environment clean.
11. Why and when should an individual wash hands? Write down the proper method of washing hand. 
Ans. Reason of washing hand
We take food with hand. We often touch our lips, face and nose with our hands. By contaminated hands, germs or toxins may enter our body through mouth, eyes and nose. Therefore, one must wash the hands to stay healthy.
Time of washing hand
Hands must be washed before [1] taking food, [2] cooking, [3] serving food, [4] feeding and attending a baby or a patient.
Proper washing of hand is a must after using toilet, cleaning raw vegetables, fish and meat, attending a patient, sweeping and cleaning rooms and toilets, polishing shoe and tying shoe-laces, checking air pressure of cycle tyre, coming home from outside, etc.
Proper method of washing hand
Proper method of washing hand is mentioned below.
[1] Wet the hands with clean water. [2] Take liquid hand-wash or soap in wet hands and rub it for one or two minutes to make rich foam. [3] Carefully rub in between all fingers. [4] Place the hands under running water and continue rubbing until the foam clears. [5] Close tap with clean cloth and wipe the hand with dry and clean towel.
12. Write a short note on ‘WASH’ programme of UNICEF.
Ans. ‘WASH’ programme of UNICEF
Percentage of healthy children in a population is the index of development of a nation. But it is unfortunate that even in the 21st century, millions of children suffer from diarrhoea and several other water-borne diseases. This happens because of contaminated water, which occurs mostly due to lack of proper sanitation. 44% of global population defecate in open areas. Faeces contain numerous germs, which easily contaminate water, especially during rainy season. In view of that, UNICEF (United Nations International Children’s Emergency Fund) has developed a global strategy to lift the standard of health of school children by providing them adequate drinking water, infrastructure for proper sanitation and sense of hygiene. This programme is popularly known as ‘WASH’ (Water Sanitation Hygiene programme). United Nations has set a goal for sustainable development of the standard of living of world population in this millennium. To reach the target, UNICEF haş developed a strategy to maximise child survival rate by providing an access to adequate drinking water, sanitation facilities to schools all over the world along with education and nutrition.
13. Describe the WASH strategy taken by UNICEF to improve hygiene behaviour among school children. How can the WASH programme be made successful? 
Ans. WASH strategy to improve hygiene behaviour among school children
Schools are the places where a child spends most of the time of his or her life all through the development period. So UNICEF has developed the plan to make every school a child-friendly place. In view of that UNICEF has extended both monetary and strategic support to all developing countries of the world to develop infrastructure for child-friendly schools. The strategy to enhance child survival and development, UNICEF has encouraged a three pillar approach. The first is enabling a child-friendly environment in the school. Next is to improve hygiene behaviour among the students and the third one is to build infrastructure for water supply and sanitation services.
Points to make WASH successful
The points to make WASH successful are as follows- [1] Wash your programme hands with adequate water and soap before taking food and after using Three pillar approach of WASH toilets. [2] Always use sanitary toilets. [3] Clean raw fruits and vegetables before consuming. [4] Keep a close vigilance on drinking water sources to reduce contamination to zero. [5] Clean water storage containers every day. [6] Keep your domestic and school toilets clean. [7] Clip your nails regularly and do not let any filth to deposit under it.
14. Mention different hygienic practices to secure good health of a person as well as of the society.
Ans. Different types of hygienic practices
The conditions or practices helpful to maintain health and to prevent diseases, especially through cleanliness are known as hygiene. Hygiene can be practiced in three different levels, i.e., personal, household and social levels, which are mentioned below.
  1. Personal level hygiene: [A] Cleaning hands before taking food, after using toilet and handing any unclean material. [B] Brushing teeth after taking meal and before going to bed. [C] Taking bath once or twice a day. [D] Trimming hair and clipping nails regularly.
  2. Household level hygiene: [A] Washing and sun-drying clothing and bedding at regular intervals. [B] Cleaning utensils, kitchen equipment, raw vegetables, fish and meat. [C] Cleaning toilet, basins and sinks. [D] Cleaning and disinfecting water reservoirs.
  3. Social level hygiene: [A] Cleaning of sewage system at regular interval. [B] Maintaining general cleanliness in hospitals, educational institutions, market places, railway stations, bus terminus, auditoriums, theaters and other community places. [C] Regular cleaning and disinfecting public toilets.

Short Answer Type Questions

1. What is immunology?
Ans. The branch of science, that deals with the study of antigens, antibodies and the overall immune system of the body, is known as immunology.
2. What is immunity?
Ans. Immunity is defined as the defence mechanism of the body against a specific infection or toxin or any harmful foreign material, entering the body by the action of specific antibodies or specialised cells or agents.
3. What is meant by innate immunity?
Ans. The innate immunity refers to non-specific defence mechanisms that come into play immediately or within hours of an antigen’s appearance in the body.
4. What is meant by acquired immunity?
Ans. The immunity, which develops within the body of an organism by any infection, vaccination or by introduction of antibody artificially, is called acquired immunity.
5. Schematically represent different types of acquired immunity.
Ans. Schematic representation of different types of acquired immunity is given below-
6. What is meant by active acquired immunity?
Ans. The immunity, which is developed in the body of an organism by introduction of an antigen, naturally or artificially, is called active acquired immunity.
7. What is artificial active immunity?
Ans. The active immunity, which develops in an organism after vaccination is called artificial active immunity. Examples-Vaccines like DPT, OPV, BCG, etc. develop artificial active immunity.
8. What is passive acquired immunity? 
Ans. The immunity which is developed in the body of an organism by introduction of an antibody, naturally or artificially, is called passive acquired immunity.
9. What is natural active immunity?
Ans. The active immunity, which is developed in an organism after natural infection by any pathogen (virus, bacteria, etc.) is known as natural active immunity. Example-After infection of chicken pox, a person develops natural active immunity against this disease and is not attacked by the same disease in future.
10. What is natural passive immunity?
Ans. The passive immunity, which is developed by the inflow of antibodies from mother to the foetus through placental circulation is known as natural passive immunity. Example-Immunoglobulin A, acquired by a baby through colostrum of breast milk of the mother, develops natural passive immunity.
11. What is artificial passive immunity?
Ans. The passive immunity, which develops in an animal by introduction of antibodies from outside by injection, is known as artificial passive immunity. Example-Antitoxin serum, produced from horse’s blood, is injected to save the life of a person suffering from snake bite.
12. What is an antigen?
Ans. Antigens are foreign materials that stimulate the immune system of the body by inducing the synthesis of specific antibodies and sensitising immunologically significant white blood cells.
13. Mention the characteristic features of antigens.
Ans. Characteristic features of antigens are as follows [1] Antigens are commonly proteins, or polysaccharides in nature, [2] Antigens are generally of high molecular weight. Minimum molecular weight of an antigen is 10000 Da. [3] An antigen induces the synthesis of a specific antibody.
14. What are the common sources of antigens?
Ans. The common sources of antigens are as follows-[1] Surface proteins of bacteria and viral capsid are the common sources of antigens. [2] Different toxins, snake venom, sting poisons of bees, scorpions act wasps, as antigens. [3] Secretory or excretory materials of bacteria or parasites contain antigens. [4] Some chemicals, drugs, pollen grains even food materials contain allergic antigens (allergens).
15. What are exogenous antigens?
Ans. The antigens, which enter into the body from outside, are called exogenous antigens. Exogenous antigens include different disease-causing germs, certain allergic food materials, chemicals, pollen grains.
16. What is endogenous antigen?
Ans. The antigenic materials, which are synthesised inside the body and are capable of inducing immune response are called endogenous antigens. Certain proteins present on the surface of RBC, cardiolipin of mammalian heart, prostate specific antigen of prostate gland are the examples of endogenous antigens.
17. What is an antibody?
Ans. Antibodies are immunoglobulin molecules, which are produced or remain in blood to counteract and inactivate specific antigens and thereby protect the body against their harmful effects.
18. What is primary immune response?
Ans. The reactions in the body in response to an antigen for the first time is called primary immune response. This response takes a little more time (10-17 days) for recognition of the antigen.
19. What is secondary immune response? |
Ans. The reactions in response to an antigen, already known to the body’s immune system, is known as secondary immune response. This response is very fast (2-7 days) because memory cells, already produced in the body, recognise the antigen and respond instantaneously.
20. What do you mean by first line of defence and second line of defence?
Ans. The first line of defence are the various types of physical and chemical barriers that prevent the entry of foreign agents into the body.
The second line of defense is a non-specific resistance, which is also considered as a function of innate immunity. The phagocytes, interferons, inflammatory reactions, natural killer cells and complement system are the components of the second line of defence.
21. What in meant by primary lymphoid organ?
Ans. The organs, within which T and B lymphocytes attain maturity, are called primary lymphoid organs. Example-Thymus gland, the site of maturity of T cells and red bone marrow, the tissue where B cells mature, are the two primary lymphoid organs.
22. What is secondary lymphoid organ?
Ans. Matured T and B lymphocytes are transferred to some other organs for further proliferation. These organs are known as secondary lymphoid organs. Example-All lymph glands, especially tonsils and spleen are the examples of secondary lymphoid organs.
23. What is meant by cell-mediated immunity?
Ans. The immune response, which involves activity of the cytotoxic T lymphocytes and different phagocytic cells in destroying the pathogens or pathogen infected cells within the body, is called cell-mediated immunity.
24. What is memory cell? State its role in Immunology.
Ans. Memory cell: Memory cells are long-lived immune cells capable of recognising foreign particles they were previously exposed to. These immune cells do not respond immediately when it first encounters an antigen but facilitates a more rapid secondary response when the antigen is encountered on a subsequent occasion.
Function: Examples of memory cells are memory B cells and memory T cells. Unlike memory B cells, the memory T cells do not produce antibodies. Reexposure to the pathogens cause them to clone themselves immediately and as such respond to the infection more strongly.
25. What do you mean by B cell and T cell?
Ans. The major cells of the immune system are the WBC’s called lymphocytes. The lymphocytes are of two types-T lymphocytes or T cells and B lymphocytes or B cells. T. lymphocytes form cell mediated immune system and B lymphocytes form humoral or antibody mediated immune system.
26. What is interferon (IFN)?
Ans. Interferon is a group of proteins that is formed and released by the host cells in response to pathogens specially virus. These are highly effective in combating hepatitis, influenza, etc.
27. What is MALT?
Ans. The full form of MALT is Mucosa Associated Lymphoid Tissue. These types of lymphoid tissues are located within the lining of respiratory tract, digestive tract and uro-genital tract and it constitutes 50% of lymphoid tissues in human body.
28. What is the main objective vaccination?
Ans. Generating memory cells in the blood to recognise a specific antigen for quick triggering of immunological response in case of infection by the same pathogen in future is the main objective of vaccination.
29. What is meant by attenuated vaccine?
Ans. The vaccines, which are prepared by living but inactivated pathogens (bacteria or virus) are called attenuated vaccines. Examples-Vaccines of tuberculosis, mumps, influenza, and Oral polio vaccine (Sabin vaccine) belong to attenuated vaccine.
30. What is meant by killed vaccine?
Ans. The vaccines, which are prepared with dead or inactivated bacteria or virus, are known as killed vaccines. Examples-Vaccines of typhoid, cholera, whooping cough (pertussis), rabies, hepatitis B and Salk vaccine belong to killed vaccine category.
31. What is a toxoid?
Ans. Toxins collected from pathogenic microorganisms, are chemically detoxified keeping their antigenic property intact. These preparations are used as vaccines, which are called toxoids. Examples-Tetanus and diphtheria vaccines are of this type.
32. What is Tetanus toxoid?
Ans. It is an inactivated form of tetanus toxin obtained from Clostridium tetani, used to produce an active immunity to the toxin.
33. What is meant by combination vaccines?
Ans. Certain vaccines are prepared with different antigens to immunise individuals against more than one disease by a single inoculation. These vaccines are called combination vaccines. Examples-DPT (Diphtheria, Pertussis, Tetanus) vaccine, MMR (Mumps, Measles, Rubella) vaccine are of this type.
34. What is booster dose of vaccine?
Ans. To maintain a steady stock of memory cells in the immune system certain vaccines are applied at regular intervals. These slots of vaccines are known as booster dose. Example-Booster dose of tetanus toxoid must be given after every ten years.
35. Why you need DPT vaccine? When should DPT vaccine be given? 
Ans. DPT vaccine is a combination vaccine used for immunisation against Diphtheria, Pertussis and Tetanus.
The standard schedule is three primary doses at 6, 10 and 14 weeks and two boosters at 15-18 months and 5 years.
36. Why you need MMR vaccine? Mention its type. 
Ans. MMR vaccine: The MMR vaccine is a vaccine against Measles, Mumps and Rubella (German measles). The first dose is generally given to children around 9-15 months of age, with a second dose at 15 months to 6 years of age, with at least 4 weeks between the doses.
Type: It is a type of live attenuated, combined vaccine.
37. Name the causative agent of diphtheria. How is the disease transmitted?
Ans. Causative agent of diphtheria: Causative agent of diphtheria is a bacterium named Corynebacterium diphtheriae.
Mode of transmission of diphtheria: Diphtheria is transmitted mainly through respiratory droplets. This disease is also spread by touching the body of an infected person and from contaminated materials like telephone, utensils, towels, handkerchiefs, etc.

Very Short Answer Type Questions

Multiple Choice Questions & Answers [MCQ]

1. The word ‘immunis’ means-
A. Exempt
B. Recovery
C. Health
D. Hygiene
Ans. A
2. Who is known as the father of immunology?
A. Louis Pasteur
B. Edward Jenner
C. Alexander Fleming
D. Robert Koch
Ans. B
3. Which of the following is not an immunological response?
A. Formation of antibody in response to antigen
B. Monocytes killing germs by phagocytosis
C. Antibody formation by vaccination
D. Killing germs by antibiotic
Ans. D
4. Which of the following is involved in immunity?
A. Lymphocyte cells
B. Plasma proteins
C. Lymph and lymph gland
D. All of these
Ans. D
5. Active immunity develops within the body by-
A. Inflow of antibodies from mother’s blood to foetal blood through placental circulation
B. Blood transfusion
C. Primary infection of any antigenic pathogen
D. Direct injection of antibodies into the body
Ans. C
6. Immunity against chicken pox that is developed by a person after first infection from the same disease is-
A. Innate immunity
B. Active acquired immunity
C. Passive acquired immunity
D. None of these
Ans. B
7. Passive immunity develops within the body by-
A. Direct injection of any attenuated germ
B. Inflow of antibodies from mother to foetus through placental circulation
C. Injection of a toxin, extracted from antigenic pathogen
D. None of these
Ans. B
8. Chemically antigen is a-
A. Polysaccharide
B. Lipoprotein
C. Glycoprotein
D. All of these
Ans. B
9. Which one of the following is not related to phagocytosis process
A. Macrophages
B. Lymphocytes
C. Neutrophils
D. Monocytes
Ans. B
10. Select the correct option from the following-
A. An IgG molecule has two antigen binding sites and one heavy and one light chain
B. IgG molecule is composed of two light and two heavy chains
C. An antigen-binding site of IgG is made up of two light chains
D. An antigen-binding site of IgG is composed of two heavy chains
Ans. B
11. The main immunoglobulin present in blood plasma is
A. IgA
B. IgG
C. IgM
D. IgD
Ans. B
12. The immunoglobulin, which reaches the body of neonates through colostrum is
A. IgA
B. IgG
C. IgM
D. IgD
Ans. A
13. Cell-mediated response involves-
A. Helper T cells
B. Plasma Cells
C. Antibodies
D. None of these
Ans. A
14. Cytotoxic T cells kill –
A. Healthy body cells
B. Infected body cells
C. Macrophages
D. All of these
Ans. B
15. T cells mature in
A. Bone marrow
B. Blood
C. Thymus gland
D. Kidney
Ans. C
16. Helper T cells activate
A. Phagocytic macrophage
B. Killer T cells
C. B cells
D. Both B and C
Ans. D
17. An antiviral substance produced within a virusinfected cell to prevent proliferation and spreading of virus is
A. Antigen
B. Antibody
C. Interferon
D. Toxin
Ans. C
18. Humoral response is related to-
A. Ts-cells
B. Tc -cells
C. Antibodies
D. None of these
Ans. C
19. In B cells ‘B’ indicates
A. B blood group
B. B agglutinogen
C. Beta antibody
D. Bursa fabricius
Ans. D
20. Which cells produce specific antibody to inactivate antigens? 
A. T lymphocytes
B. RBC
C. Plasma cells
D. Macrophages
Ans. C
21. Formation of antibody depends directly upon-
A. Cytotoxic T cells
B. Helper T cells
C. Monocytes
D. Plasma cells
Ans. D
22. Memory cells, involved in immune response are formed by-
A. Neutrophils
B. Basophils
C. Monocytes
D. Lymphocytes
Ans. D
23. Memory cells are stored in- 
A. Brain
B. Liver
C. Spleen
D. Kidney
Ans. C
24 Which of the following is present on the cell membrane of mast cells and B lymphocytes?
A. IgE
B. IgG
C. IgM
D. IgA
Ans. A
25. The names of Sara Nelmes and James Phipps are related to
A. Small pox vaccine
B. Chicken pox vaccine
C. Cholera vaccine
D. Cow pox vaccine
Ans. A
26. Effectiveness of a vaccine is retained within the body by
A. Helper T cells
B. Macrophage cells
C. Memory T and B cells
D. All lymphocytes
Ans. C
27. A disease, that has been completely eradicated by vaccination is
A. Diphtheria
B. Small pox
C. Chicken pox
D. Polio
Ans. B
28. Small pox was declared officially as an eradicated disease-by WHO in the year-
A. 1977
B. 1978
C. 1796
D. 1980
Ans. D
29. In attenuated vaccine-
A. Dead germs are used
B. Living but inactive germs are used
C. Toxins from the germs are used
D. Combination of bacteria and viruses are used
Ans. B
30. Killed germs are used to immunise against-
A. Tetanus
B. Plague
C. Diphtheria
D. All of these
Ans. B
31. DPT is a-
A Attenuated vaccine
B. Inactive vaccine
C. Toxoid
D. Combination vaccine
Ans. D
32. BCG vaccine is applied to immunise babies against
A. Polio
B. Whooping cough
C. Tuberculosis
D. Cholera
Ans. C
33. Orally administrable vaccine
A. Salk polio vaccine
B. Sabin polio vaccine
C. Cholera vaccine
D. Rubella vaccine
Ans. A
34. Diarrhoea may be caused by-
A. Entamoeba
B. Shigella
C. Salmonella
D. All of these
Ans. D
35. Which of the following diseases may be caused by protozoa, bacteria and even virus?
A. Cholera
B. Diphtheria
C. Pneumonia
D. Diarrhoea
Ans. D
36. Prolonged diarrhoea results into-
A. Severe respiratory distress
B. Dehydration of the body
C. Very high fever
D. Severe headache
Ans. B
37. The organism acting as a vector for diarrhoea causing pathogen is
A. House Fly
B. Cockroach
C. Both
D. None of these
Ans. A
38. Splenomegaly or enlargement of spleen is a common symptom of
A. Tuberculosis
B. Malaria
C. Hepatitis B
D. Dengue
Ans. B
39. Feeling of extreme chill followed by very high fever is the symptom of
A. Pneumonia
B. Diphtheria
C. Malaria
D. Cholera
Ans. C
40. Plasmodium, the pathogen of malaria is a type of
A. Bacteria
B. Virus
C. Fungus
D. Protozoa
Ans. D

Answer in a single word or sentence

1. What is the name of the reaction shown by the body against any pathogen?
Ans. The reaction shown by the body against any pathogen is commonly called immune response.
2. What are pathogens?
Ans. A pathogen is a disease causing microorganism.
3. Which type of immunity develops by birth?
Ans. Innate immunity develops by birth.
4. Which type of immunity is provided by neutrophils?
Ans. Neutrophils provide innate immunity to the body.
5. Which characteristic ability of antigen activates the immune system of the body?
Ans. Immunogenicity of antigen activates the immune system of the body.
6. Which characteristic ability of antigen makes it compatible to the antibody?
Ans. Antigenicity of an antigen makes it compatible to the antibody.
7. Which is the first line of defence in our immune system?
Ans. Skin and mucous membrane provide the first line of defence in our body’s immune system.
8. Which is the second line of defence in our immune system?
Ans. Phagocytic white blood cells like neutrophils and monocytes provide the second line of defence in our body’s immune system.
9. Which is the third line of defence of human immune system?
Ans. Acquired immunity provided by different types of T and B lymphocytes provides the third line of defence to human immune system.
10. What is the name of the chemical, which is produced inside the body to resist a toxin?
Ans. Antitoxin is produced inside the body to resist a toxin.
11. How does an immunoglobulin G molecule appear?
Ans. Immunoglobulin G molecules appears like English letter ‘Y’.
12. Which is the most abundant antibody present in human blood?
Ans. Immunoglobulin G is the most abundant antibody present in human blood.
13. Which type of antibody is present in different body fluids and secretory materials in human body?
Ans. Immunoglobulin A is present in different body fluids and secretory materials in the human body.
14. Which is the largest antibody present in our body?
Ans. Immunoglobulin M is the largest antibody present in our body.
15. In which antibody five molecules stay as a cluster?
Ans. In immunoglobulin M, five molecules stay as a cluster.
16. Where do B lymphocytes mature?
Ans. B lymphocyte or B cells mature in red bone marrow.
17. How many globulin chains combine to prepare an immunoglobulin G molecule?
Ans. Two heavy and two light globulin chains combine to prepare an immunoglobulin G molecule.
18. How do the globulin chains remain tied together in an immunoglobulin G molecule?
Ans. In an immunoglobulin G molecule the heavy and light chains remain tied together by disulphide bonds.
19. Which type of antibody can penetrate the placental barrier to reach the foetal blood from mother’s body?
Ans. IgG can penetrate the placental barrier to reach the foetal blood from mother’s body.
20. Which antibody is present in tears?
Ans. IgA is present in tears.
21. Which antibody is synthesised first in human foetus?
Ans. The antibody synthesised first in human foetus is IgM.
22. Which antibody protects our body against allergens?
Ans. IgE protects our body against allergens.
23. What are allergens?
Ans. The substances which trigger allergies are called allergens.
24. What is meant by vaccination?
Ans. Inoculation of the body with vaccines to develop active acquired immunity against specific disease-causing germs or toxins is called vaccination.
25. What is the full form of DPT vaccine?
Ans. Full form of DPT is Diphtheria, Pertussis (whooping cough), Tetanus.
26. What is the full form of TT?
Ans. Full form of TT is Tetanus toxoid.
27. Which vaccine did Calmette and Guerin discover?
Ans. Calmette and Guerin discovered the vaccine against tuberculosis.
28. Who discovered the vaccine of polio?
Ans. Jonas Salk discovered the vaccine of polio.
29. Who discovered the Oral Polio Vaccine?
Ans. Albert Sabin discovered the Oral Polio Vaccine.
30. What is the full form of BCG?
Ans. Full form of BCG is Bacillus Calmette-Guerin.
31. What is the full form of MMR?
Ans. Full form of MMR is Mumps-Measles-Rubella.
32. What is the full form of ATS?
Ans. Full form of ATS is Anti-Tetanus Serum.
33. What is the full form of OPV?
Ans. Full form of OPV is Oral Polio Vaccine.
34. Give example of attenuated viral vaccines.
Ans. Vaccines of mumps, measles, rubella, chicken pox belong to attenuated viral vaccines.
35. What is sub-unit vaccine?
Ans. The vaccine which is prepared by certain molecular part of a pathogen, a viral protein for instance, is called sub-unit vaccine.
36. Give example of a sub-unit vaccine.
Ans. Vaccine of Hepatitis B is a sub-unit vaccine.
37. To which kingdom of the living world does malaria pathogen belong?
Ans. Malaria pathogen belongs to kingdom Protista.
38. What is the other name of ‘Break Bone fever’?
Ans. The other name of ‘Break Bone fever’ is dengue.
39. Which disease is commonly known as ‘Lock Jaw’ disease?
Ans. Tetanus is commonly known as ‘Lock Jaw disease’.
40. Name a viral disease which is transmitted by mosquito.
Ans. Dengue is a viral disease which is transmitted by mosquito.
41. Which type of hepatitis is transmitted through contaminated food and drink?
Ans. Hepatitis A is transmitted through contaminated food and drink.
42. Name a virus, which causes severe diarrhoea.
Ans. Rotavirus causes severe diarrhoea.
43. Name a disease caused by retrovirus.
Ans. AIDS is caused by retrovirus.
44. What is full form of AIDS?
Ans. Full form of AIDS is Acquired Immune Deficiency Syndrome.
45. Which day is observed as World AIDS Day?
Ans. Every year 1st December is observed as the World AIDS Day.

Fill in the blanks

1. The ability of body to resist harmful effect of pathogens is known as ……….
Ans. Immunity
2. …….. system is formed for protecting our body from the hands of the foreign invaders.
Ans. Immune
3. Acquired ……….. immunity develops naturally due to the introduction of antigens.
Ans. Active
4. Antibody is produced in the body by the influence of …………
Ans. Antigen
5. Thymus gland is a ……….. lymphoid organ.
Ans. Primary
6. There are ……….. types of antibody in our body.
Ans. 5
7. Antibody is ……….. shaped.
Ans. Y
8. Antibody is made up of ………… polypeptide chain.
Ans. 4
9. In ‘T’ cells, ‘T’ indicate ………….
Ans. Thymus
10. ……….. cells destroy TC cells and TH cells.
Ans. Suppressor
11. Plasma cells produce specific antibodies to inactivate ………..
Ans. Antigen
12. Two types of B cells are plasma cells and ………… cells.
Ans. Memory
13. Memory cells stay in ………… glands.
Ans. Lymph
14. An ……….. is a substance that can cause an allergic reaction by histamine secretion.
Ans. Allergen
15. ………… is the founder of vaccinology.
Ans. Edward Jenner
16. The first small pox vaccine was discovered by ………..
Ans. Edward Jenner
17. …………. vaccine introduced by Calmette Guerin.
Ans. BCG
18. BCG vaccine is a type of ……….. vaccine.
Ans. Attenuated
19. Small pox vaccine is a ………. vaccine.
Ans. Heterotypic
20. Tetanus vaccine is prepared by inactivated ……….. from Clostridium tetani.
Ans. Toxin

State True or False

1. Antigens mainly include proteins.
Ans. True
2. Toxic chemicals like snake venom can act as antigens.
Ans. True
3. Epitope is a part of antibody.
Ans. False
4. IgM is a pentamer.
Ans. True
5. IgA is present in tears.
Ans. True
6. Vaccination develops artificial active immunity.
Ans. True
7. Vaccine against Hepatitis B is an example of subunit vaccine.
Ans. True
8. Stiffness of muscles with severe pain is a symptom of diarrhoea.
Ans. False
9. Tuberculosis is a virus borne disease.
Ans. False
10. Yellowing of skin and eye is a symptom of pneumonia.
Ans. False

TOPIC – 2

MICROBES IN HUMAN WELFARE

SUMMARY

  • The microbes or microorganisms are the organisms which are not visible with naked eyes but can be seen only under the microscope.
  • Different virus, bacteria, protozoa and insects can reduce the population growth of various insect pests of crops by causing disease, parasitism and predation.
  • Integrated Pest Management (IPM) has been developed in view of sustainable development of agriculture.
  • Organisms that enrich the nutrient quality of the soil are called biofertilisers.
  • Like nitrogen fixing bacteria, many species of cyanobacteria can fix atmospheric nitrogen.
  • There are some fungi which live in close association with the roots of some plants and live symbiotically. Such symbiotic association between fungi and plant roots is known as mycorrhiza.
  • Biofertilisers are eco-friendly and cost-effective.

Long Answer Type Questions

1. Give a brief account of different microbial bio-control agents.
Ans. Different microbial bio-control agents
Different bacteria, virus, fungi and protozoa can be used as bio-control agents.
  1. Bacteria as bio-control agent: Bacillus thuringiensis produces a poison, called Bt toxin, which is used to control growth of larvae of moths, beetles and flies. Scientists have introduced genetic materials of these bacteria in corn, vegetables and cotton plants. As a result, Bt-toxin is synthesized in these plants, so that insect parasites cannot attack them. Pseudomonas fluonescena has been commercially used as fungicide. Bacillus popillae is used to control Japanese beetle, a pest of maple and rose plants.
  2. Virus as bio-control agent: [A] Caudovirales is a group of phage virus, which kill Salmonella (bacteria) from water bodies. This phage virus can be used to eliminate Salmonella from drinking water to protect us from severe diarrhoea. [B] Nuclear Polyhedrosis Virus (NPV) or Cytoplasmic Polyhedrosis Virus (CPV) can kill larvae of moths and butterflies. These viruses are used to destroy some harmful insects like pine sawflies, gypsy moths, tussock moths, etc.
  3. Protozoa as bio-control agent: [A] Nosema locustae is a protozoan microbe, which is used to control some insect pests. [B] Mattesia grandis, another protozoa, effectively controls cotton ball weevils.
  4. Fungus as bio-control agent: Beauveria bassiana is a fungus, which can control mosquito population effectively. It can be used in those areas, where mosquitoes have developed resistance against chemical insecticides. It can also control termites, whiteflies and many other insects.
2. What is meant by biofertiliser? Mention the roles of bacteria as biofertiliser. 
Ans. Biofertiliser
Certain microorganisms, have the ability to promote plant growth by increasing the supply or availability of primary nutrients to plants. These are applied in crop fields to increase soil fertility. These are known as biofertilisers.
Roles of different bacteria as biofertilisers
[1] Rhizobium leguminosarum is a symbiotic bacteria present in the root nodules of leguminous plants. These bacteria trap atmospheric nitrogen and convert it into nitrates. Ultimately this nitrate is transferred to the plant body as an essential nutrient. [2] Frankia is another symbiotic bacteria, which dwell in the root nodules of plants like Casuarina, Rubus, Alnus, etc. [3] Some soil-living, aerobic bacteria can absorb atmospheric nitrogen into the cells. After death, this nitrogen is released in soil as nitrates and increases its fertility. Azotobacter sp., Azomonas sp., Derxia sp., Azospirillum sp., etc., are some examples. [4] Among the anaerobic nitrogen-fixing bacteria Clostridium sp., Chromatium sp., Chlorobium sp. are mentionable. [5] Some bacteria like Thiobacillus, Micrococcus, Acrobacter are capable of solubilizing inorganic phosphorus from insoluble compounds in the soil. Use of these bacteria as biofertilisers enhance phosphate-nutrition of plants.
3. What do you mean by nitrogen fixation? Mention the roles of cyanobacteria as biofertiliser.
Ans. Nitrogen fixation
Nitrogen fixation is a process in which nitrogen (N2) in the atmosphere is converted into ammonia (NH3). Nitrogen fixation process occurs naturally in the soil by nitrogen-fixing bacteria.
Roles of cyanobacteria as biofertilisers
Some cyanobactería can trap atmospheric nitrogen. These include Anabaena sp., Nostoc sp., Aulosira sp., Stigonema sp., Plectonema sp., etc. These are now cultured in water of paddy fields. When these organisms die and decompose, nitrates get absorbed in soil and increase its fertility. Recently Azolla is being cultivated in the irrigated paddy fields. Azolla is an aquatic fern. Nitrogen-fixing cyanobacteria Anabaena azollae live in the leaves of this fern. These cyanobacteria fix nitrogen from atmosphere and supply it to Azolla. Azolla die and get decomposed in the soil. By this process soil get nitrogen from the decomposed body of this fern. In Southeast Asian countries, cultivation of Azolla in paddy fields is a regular practice now. It has reduced the need for chemical fertilisers and has cut down the cost of paddy cultivation. Beside this, cyanobacteria secrete ascorbic acid, Vit. B and auxin in the soil.
4. Mention the roles of mycorrhiza as a bio-fertiliser. Why are biofertilisers and bio-control agents getting more acceptance all over the world? 
Ans. Roles of mycorrhiza as bio-fertiliser
Mycorrhiza is a symbiotic association between roots of some vascular plants and some fungi. In this association, the fungi develop hyphae (fine filaments of fungal vegetative body) around the root-tip or inside the cortex of roots of the vascular plants. The fungal hyphae can absorb phosphorus and nitrogenous nutrients from soil very efficiently. It can also remove harmful heavy metals like lead, mercury, etc., from the soil to reduce soil pollution. Mycorrhiza is formed in oaks, pines, orchids and some crop plants. The fungi involved in the formation of mycorrhiza are Boletus sp., Amanita sp., Glomus, etc.
Reasons behind acceptance of biofertilisers and bio-control agents
Chemical fertilisers are expensive. Production of these fertilisers need large infrastructure and power. Chemical fertilisers reduce natural soil fertility and change soil pH. Same is applicable in case of pesticides, these are very expensive and all of these materials increase environmental pollution. However, in comparison with chemical fertiliser, production cost of biofertilisers is negligible. Pest control by biological agents do not need any infrastructure at all. Production of bio-fertiliser does not need heavy infrastructure and power. Moreover bio-control is an eco-friendly procedure and biofertilisers do not cause soil pollution. Bio-fertiliser and pest control by biological agents not only retain the natural resources of earth, but also prevent them from getting amalgamated with chemical precipitations. So biofertilisers and bio-control agents are getting more acceptance all over the world.
5. What do you mean by biological pest control? Mention its advantages and disadvantages. 
Ans. Biological pest control
The control of pests with the aid of biological agents (bacteria, virus, protozoa, etc.) is known as biological pest control. Example-Use of bacteria, birds to control pest moths.
Advantages
[1] Biofertilisers do not harm non-target organisms, they are very specific. [2] No toxic chemicals are used in their preparation, hence, there are no residues of such chemicals left in soil or water. [3] No bio-accumulation of chemicals in food chains occur due to use of biofertilisers. [4] Long term usage of biofertilisers does not generate any resistance in pests.
Disadvantages
[1] Low level of pests has to be sustained for the maintenance of control agents. [2] Development and research of appropriate control agents is expensive and lengthy.
6. Write different characteristics or properties of bio-fertiliser. Mention the advantages and blo-fertilliser. Mention disadvantages of biofertilisers. 
Ans. Characteristics or properties of biofertilisers
[1] Biofertilisers contain living microorganisms. [2] They improve soil texture and yield of plants. [3] They do not allow pathogens to flourish. [4] They are eco-friendly and cost-effective. [5] They protect the environment from pollutants.
Advantages of biofertilisers
[1] They are cost-effective. [2] They supplement chemical fertilisers. [3] They are eco-friendly. [4] They reduce the cost towards the use of fertilisers, especially regarding nitrogen and phosphorus. [5] They improve texture, structure and water holding capacity of soil. [6] There are no adverse effects on plant growth and soil fertility due to the usage of biofertilisers.
Disadvantages of biofertilisers
[1] Biofertilisers require special care for long term storage because they are alive. [2] They must be used before their expiry date. [3] If other microorganisms contaminate the carrier medium or if a wrong strain is used, they are not as effective. [4] They lose their effectiveness if the soil is too hot or dry.

Short Answer Type Questions

1. What are microbes? Give example.
Ans. Microscopic organisms (less than 0.1 mm in diameter) present in air, water and soil are commonly known as microbes.
Example-Bacteria, protozoa, etc.
2. Which organisms are called bacteria?
Ans. Prokaryotic microbes, having cellular diameter less than 3 μ m with cell wall typically made up of peptidoglycan and cytoplasm carrying a circular DNA and scattered 70S ribosomes are called bacteria. Example-Bacillus, Salmonella, etc.
3. Which organisms are called protozoa?
Ans. Eukaryotic, unicellular microorganisms, carrying well-defined nucleus with chromosomes and membrane-bound cell organelles, are known as protozoa. Example-Amoeba, Plasmodium, etc.
4. What is meant by bio-control agents?
Ans. Different virus, bacteria, protozoa and insects can reduce the population growth of various insect pests of crops significantly, by causing disease, parasitism and predation. These biological agents are commonly called bio-control agents.
5. What is meant by biological control?
Ans. The technique by which abundance of pests and harmful organisms is controlled by application of their specific natural enemies is known as biological control.
6. Name two bacterial and two cyanobacterial species, commonly used as biofertilisers.
Ans. The two bacterial species commonly used as biofertilisers are Rhizobium and Azotobacter. The two cyanobacterial species commonly used as biofertilisers are Anabaena and Nostoc.
7. What is meant by GMO?
Ans. Full form of GMO is Genetically Modified Organism. By application of modern biotechnology, in recent times, plants and animals are produced with tailor made features. For this purpose, unwanted genes of the organisms are replaced by desired genes, from outer source. Such organisms show desired features, and are popularly called genetically modified organisms.
8. What are viruses?
Ans. Viruses are ultra-microscopic, nucleoproteinated, acellular intermediate entity between living and non-living. Example-HIV, Rotavirus, etc.
9. What is biomagnification?
Ans. The chemical fertilisers, insecticides, pesticides, which are used in conventional farming, enter into the food chain through producers. As these toxic chemicals pass along food chain from lower to higher trophic levels, the concentration increases step-by-step. This biological event is known as biomagnification. Biomagnification results into a number of diseases amongst the members of the higher trophic levels.
10. What is bloaccumulation? 
Ans. Bioaccumulation refers to accumulation of toxic substance in an organism. It occurs when an organism absorbs a toxic substance at a rate faster than that at which the substance is lost by catabolism or excretion.
11. How do GMO help in bio-control of insect pests?
Ans. Bacillus thuringiensis can produce a toxin, called Bt-toxin, which. fatal for sect pests. Biothe technologists have singled out toxinproducing gene from the bacterium and placed it in the plant cells. Such GMO or Genetically Modified Crop plants synthesise the Bt-toxin, which potentially kill insect pests feeding on it.
12. Mention the role any of two viruses as biocontrol agents.
Ans. [1] Nuclear Polyhedrosis Virus can potentially kill the larvae of moths and beetles. [2] Granulosis virus destroys larvae of Spodoptera litura, an insect pest.
13. Mention the role of Caudovirales in controlling water pollution.
Ans. Caudovirales is a group of phage viruses which kill Salmonella (bacteria) from water bodies. This phage virus can be used to eliminate Salmonella from drinking water to protect us from severe diarrhoea.
14. Mention the roles of any two protozoa as blocontrol agents.
Ans. [1] Nosema locustae is a protozoan microbe, which is used to control some insect pests.
[2] Mattesia grandis, another protozoa, effectively controls cotton ball weevils.
15. Mention the role of a symbiotic bacterium as biofertiliser. 
Ans. Rhizobium leguminosarum is a symbiotic bacterium present in the root nodules of leguminous plants. These bacteria trap atmospheric nitrogen and convert it into nitrates. Ultimately this nitrate is transferred to their plant partners as an essential nutrient.
16. What is Azolla? Mention its importance.
Ans. Azolla: Azolla is an aquatic floating fern which is commonly known as ‘mosquito fern!
Importance of Azolla: Azolla leaves give shelter to a nitrogen-fixing cyanobacteria named Anabaena azollae. Azolla is gown in water of paddy fields, Anabaena absorbs nitrogen from air. When water gets absorbed, Azolla decays and nitrogen gets mixed in soil to increase its fertility.
17. Why Azolla is an effective biofertiliser for paddy fields but not effective in wheat and vegetable fields?
Ans. Azolla grows in water. Paddy plants also grow in wet soil. So, Azolla is grown in water of paddy fields. On the other hand, wheat and vegetables grow in drier soil. Therefore, application of Azolla as biofertiliser is not effective for wheat and vegetable cultivation.
18. How do bacteria increase soll fertility?
Ans. Bacteria can increase soil fertility in two different ways. One type of bacteria increases nitrogen level in soil by trapping atmospheric nitrogen. While another type, makes soil-borne phosphorus, soluble and absorbable to root system.
19. Now Azotobacter can be applied in a crop be applied in a crop field as blofertiliser?
Ans. Agricultural laboratories prepare mixture of live Azotobacter cells and charcoal powder. This mixture can be applied in fields during soil preparation in the form of dust. This powder can also be mixed in water to prepare a bacterial solution. The seeds or roots of saplings may be given a dip in it before sowing and plantation.
20. Now de phosphobacteria act as biofertiliser?
Ans. Soil contains some phosphate salts, which are not absorbable by roots. Phosphobacteria make these salts soluble and absorbable to root system of plants. Thus, phosphobacteria act as a biofertiliser. Example-Bacillus megaterium is a phosphobacterium.
21. How do different biofertilisers work?
Ans. Nitrogen-fixing bacteria and cyanobacteria propagate in soil and absorb atmospheric nitrogen, which ultimately mix in soil to make it fertile. Phosphobacteria convert phosphate salts into soluble and absorbable forms. Mycorrhiza removes. harmful heavy metals (Pb, Cu, Hg, Cd, etc.) from soil to reduce soil pollution and promotes plant growth. Certain microorganisms remove weeds.
22. Mention the role of Bacillus thuringiensis as ble-control agent.
Ans. Bacillus thuringiensis produces a toxin, called Bt-toxin, which can destroy larvae of many insect pests. In recent days Bt-toxin, popularly known as ‘thuricide’ is commercially prepared and applied in cotton and vegetable crop fields to control caterpillar and grub.
23. What is the full form of IPM? Mention its objective.
Ans. Full form of IPM: Integrated Pest Management.
Objective of IPM: The concept of Integrated Pest Management has been developed in view of sustainable development of agriculture. Its main objective is to reduce the use of chemical fertilisers, insecticides, herbicides, fungicides by using biocontrol agents or protect the future generation from harmful effects of these harsh chemicals and to keep the world as much pollution free as possible.
24. Mention the advantages of bio-control or biological control of pests.
Ans. The advantages of biological control of pests are as follows-[1] Bio-control is more eco-friendly, because it does not pollute environment. [2] In biocontrol, only the target pests are killed, the crop plant and other organisms remain unharmed. [3] This method is less expensive, therefore, reduces the production cost of crops.
25. Define mycorrhiza.
Ans. Mycorrhiza is a symbiotic relationship between fungi and root systems of vascular plants. Mycorrhiza is formed in plants like pine, orchids, etc. Some of the fungi involved in the formation of the mycorrhiza are Glomus, Boletus, etc.
26. Describe the importance of VAM as bio-fertiliser.
Ans. VAM or Vesicular Arbuscular Mycorrhiza is the most popular bio-fertiliser that enhances phosphorus, sulphur and copper absorption by root systems of plants with which it is associated. VAM helps the roots of a vasular plant to capture nutrients from the soil.
27. Mention the disadvantages of bio-control of pests.  
Ans. The disadvantages of bio-control of pests are-[1] Many organisms used as bio-control agents cannot adapt themselves with the new environment and often die. [2] The effectiveness of a bio-control agent in controlling a pest cannot be assumed before its application.
28. Define chemical fertiliser.
Ans. A chemical fertiliser is defined as any inorganic material of wholly or partially synthetic origin that is added to the soil to sustain plant growth. Example-urea.
29. Define bio-fertiliser.
Ans. A bio-fertiliser is a substance which contains living microorganisms which when applied to the seeds, plant surfaces or soil promotes growth by increasing the supply or availability of primary nutrients (NPK) to the host plant.
30. Define vermiculture.
Ans. Vermiculture is the process in which earthworms are reared in a portion of soil to prepare organic manure to enhance the productivity of soil.
31. Why Azotobacter is called benificial bacteria?
Ans. Azotobacter (nitrogen fixing bacteria) convert atmospheric nitrogen into nitrogenous compounds. They are able to pick up free atmospheric nitrogen and fix it in some organic compounds like amino acids. That is why Azotobacter is called beneficial bacteria.
32. Write the role of cyanobacteria as bio fertilisers. 
Ans. There are several free living cyanobacteria such as Anabaena, Nostoc, Aulosira which can fix nitrogen. Aulosira fertilisima is known to be active nitrogen fixer in the paddy field. Cylin drospermum licheniforme grow in the field of maize and sugarcane, fixing nitrogen in the soil.
In some plants cyanobacteria exhibit symbiotic association and promote nitrogen fixation. Association between Azolla and Anabaena is the example of such an association.
33. Write about Bt-toxin.
Ans. Bacillus thuringiensis (Bt) is a Gram positive, spore-forming bacterium that synthesises parasporal crystalline inclusions containing cry and cyt proteins, which are toxic against a wide range of insect orders, nematodes.

Very Short Answer Type Questions

Multiple Choice Questions & Answers [MCQ]

1. Microbes are-
A. Virus
B. Bacteria
C. Protozoa
D. All of these
Ans. D
2. The term ‘microbe’ was coined by
A. Robert Hook
B. Sedillot
C. Robert Brown
D. Leeuwenhoek
Ans. B
3. Azolla maintains symbiotic association with
A. Azotobacter
B. Clostridium
C. Nostoc
D. Anabaena
Ans. D
4. Which is not a bio-fertiliser?
A. Rhizobium
B. Azolla
C. Agaricus
D. Azotobacter
Ans. C
5. Some cyanobacteria are used as bio-fertiliser because
A. They are capable of trapping free O2 more than other plants
B. They can solubilise phosphorus
C. They can trap atmospheric nitrogen
D. They help the soil to retain more water
Ans. C
6. ‘Bt’ for Bt-cotton stands for
A. Biologically transformed
B. Biotechnology
C. Bacillus thuringiensis
D. None of these
Ans. C
7. Mycorrhiza is an-
A. Association of fungi and algae
B. Association of fungi and flowering plants
C. Association of fungi and bacteria
D. Association of algae and vascular plants
Ans. B
8. Cyanobacteria are-
A. Chemosynthetic bacteria
B. Photosynthetic prokaryotic microorganisms
C. Non-photosynthetic nitrogen-fixing prokaryotic microorganisms
D. Nitrogen-fixing protozoa
Ans. B
9. A soil-dwelling nitrogen-fixing bacterium is-
A. Anabaena
B. Salmonella
C. Clostridium
D. Lactobacillus
Ans. C
10. Which of the following microorganisms can kill insect pests?
A. Bacillus thuringiensis
B. Leptomonas
C. Nosema locustae
D. All of these
Ans. D
11. The virus used in bio-control of insect pests is-
A. Phage virus
B. Rubella virus
C. Nuclear Polyhedrosis Virus
D. Lipovirus
Ans. C
12. An anaerobic nitrogen-fixing non-symbiotic bacterium-
A. Azotobacter
B. Clostridium
C. Rhizobium
D. Azospirillum
Ans. B
13. VAM is a/an-
A. Ectomycorrhiza
B. Ectendomycorrhiza
C.Endomycorrhiza
D. Cyanobacterium
Ans. C
14. Granulosis virus and NPV are similar as both act as-
A. Antibacterial substance
B. Bio-control agents
C. Biofertilisers
D. Disease causing agent for human
Ans. B
15. Bacteria used for controlling Japanese beetle-
A. Bacillus thuringiensis
B. Bacillus popilae
C. Bacillus subtilis
D. Rhizobium fascioli
Ans. B
16. Spodopterin, a bio-control agent for insect pest is composed of
A. Virus
B. Bacteria
C. Protozoa
D. Fungus
Ans. D
17. Example of cyanobacteria is-
A. Anabaena
B. Gleocapsa
C. Oscillatoria
D. All of these
Ans. D
18. Mycorrhizae fungi develop in symbiotic association with
A. Root system of pine
B. Leaf of fern
C. Green algae
D. None of these
Ans. A
19. Application of Azolla in paddy fields are popularly practiced in
A. European countries
B. South American states
C. Southeast Asian countries
D. All of these
Ans. C
20. Mycorrhiza is used as a bio-fertiliser because it-
A. Can remove harmful heavy metals from soil
B. Can absorb water
C. Can help in mineral absorption
D. All of these
Ans. D
21. An antibiotic producing fungus is-
A. Aspergillus
B. Agaricus
C. Penicillium
D. Saccharomyces
Ans. C
22. Nitrifying bacteria are-
A. Parasitic bacteria
B. Chemosynthetic bacteria
C. Autotrophic bacteria
D. None of these
Ans. B
23. Bacteria which are capable of trapping atmospheric nitrogen are-
A. Putrefying bacteria
B. Nitrogen-fixing bacteria
C. Denitrifying bacteria
D. Nitrifying bacteria
Ans. B
24. The bacterium, mostly used in the experiments of biotechnology is-
A. Rhizobium
B. Streptomyces
C. Salmonella
D. E.coli
Ans. D
25. Rhizobium is a-
A. Free-living, nitrogen-fixing bacteria
B. Symbiotic, nitrogen-fixing bacteria
C. Cyanobacteria
D. Denitrifying bacteria
Ans. B
26. Application of Azolla in paddy field increases …….. in soil.
A. Phosphorus
B. Nitrogen
C. Sulphur
D. Potassium
Ans. B
27. Nostoc is a –
A. Nitrogen-fixing protozoa
B. Nitrogen-fixing cyanobacteria
C. Symbiotic fungus
D. Green algae
Ans. B
28. Which of the following organisms is not used as bio-fertiliser?
A. Rhizobium
B. Lichen
C. Azotobacter
D. Azolla
Ans. B
29. Insoluble phosphate of soil is transformed into soluble phosphate by-
A. Azolla
B. Nostoc
C. Rhizobium
D. Pseudmonas putida
Ans. D
30. Sweet pea plant can grow in infertile land because of-
A. Azotobacter
B. Clostridium
C. Rhizobium
D. Anabaena
Ans. C

Answer in a single word or sentence

1. Who is known as the ‘Father of microbiology’?
Ans. Louis Pasteur is known as the ‘Father of microbiology
2. Give an example of a species of bacteria that is used for biological control of insect pest?
Ans. Bacillus thuringiensis
3. Name a symbiotic bacterium, which can trap atmospheric nitrogen for its plant partner.
Ans. Rhizobium leguminosarum
4. Which soil-living bacterium is applied in crop fields to increase nitrogen content of the soil?
Ans. Azotobacter
5. Name a common viral bio-control agent against insect pest.
Ans. Nuclear Polyhedrosis Virus
6. Crystalline protein obtained from which bacterium is popularly known as ‘cry protein’?
Ans. Bacillus thuringiensis
7. Which bacterium is used to kill the larvae of Anopheles mosquito?
Ans. Bacillus sphaericus
8. Name a bacterial species dwelling in the root nodules of leguminous plants.
Ans. Rhizobium leguminosarum
9. Name an endomycorrhiza, used as bio-fertiliser.
Ans. VAM is an endomycorrhiza used as bio-fertiliser.
10. Name two fungal associates of mycorrhiza.
Ans. Amanita and Boletus are two common fungal associates of mycorrhiza.
11. How does mycorrhiza help in reducing soil pollution?
Ans. Mycorrhiza helps in reducing soil pollution removing harmful heavy metals like lead, mercury, etc., from soil.
12. What is the full form of VAM?
Ans. Vesicular Arbuscular Mycorrhiza
13. Which types of mycorrhiza are found in nature?
Ans. Three different types of mycorrhiza are found in nature, these are-ectomycorrhiza, endomycorrhiza and ectendomycorrhiza.
14. Give examples of two fungi, which form ectomycorrhiza.
Ans. Boletus and Amanita
15. Give examples of two fungi, which form VAM or endomycorrhiza.
Ans. Glomus and Acaulospora
16. Give examples of two fungi, which forms ectendomycorrhiza.
Ans. Clavaria and Endagon
17. Name two plants in which ectomycorrhiza grows on the roots.
Ans. Oak and pine
18. What is the full form of NPV?
Ans. Nuclear Polyhedrosis Virus
19. What is the full form of CPV?
Ans. Cytoplasmic Polyhedrosis Virus
20. Mention of the importance of NPV and CPV as bio-control agent.
Ans. NPV and CPV are used as Bio-control agents to destroy several caterpillar pests of vegetable plants.
21. What is an antibiotic?
Ans. Antibiotic is a chemical substance that kills bacteria, secreted by microorganisms which can kill the pathogens. Examples-Penicillin and streptomycin.
22. Which type of phage virus is used to kill Salmonella bacteria in water bodies?
Ans. Caudovirales is a group of phage virus used to control Salmonella build-up in water bodies.
23. Name a protozoan microbe, used to control insect pest.
Ans. Nosema locustae

Fill in the blanks

1. Bacillus thuringiensis produces a poison called ……….
Ans. Bt-toxin
2. Rhizobium is present in the ………… nodules of leguminous plants.
Ans. Root
3. Mycorrhiza exhibits the phenomenon of ………..
Ans. Symbiosis
4. Beauveria bassiana is a fungus, which controls ……….. pest population effectively.
Ans. Insect
5. Mycorrhizal ………… absorb nutrients from soil very efficiently.
Ans. Hyphae
6. ……….. is a protozoan microbe which effectively controls cotton ball weevils.
Ans. Mettasia grandis
7. Anabaena is present in the ………… cavities of Azolla.
Ans. Leaf
8. Agriculture by using only biofertilisers is called ………….
Ans. Organic farming
9. Cyanobacteria serves as important biofertilisers in the fields of ………..
Ans. Paddy
10. One of the free-living anaerobic nitrogen-fixer is ………..
Ans. Rhodospirillum
11. Bacillus thuringiensis is widely used as …………
Ans. Insecticide
12. ………… is a pigment that gives a pinkish hue to rhizobium induced root nodules.
Ans. Leg haemoglobin
13. Antibiotics are the most effective on …………
Ans. Bacteria
14. A nitrogen fixing microbe associated with the fern Azolla in rice fields is …………
Ans. Anabaena
15. Azolla pinnata has been found to be an important bio-fertiliser for paddy crops. The quality is due to the presence of …………
Ans. N2 fixing bacteria
16. Non-symbiotic bio-fertiliser is …………
Ans. Azotobacter
17. IPM (Integrated Pest Management) discourages the excessive use of …………
Ans. Chemical pesticides
18. ………… used as bio-fertiliser in cotton field.
Ans. Azotobacter chroococcum
19. The symbiotic association between fungi and roots of higher plants is referred to as …………
Ans. Mycorrhiza
20. Baculoviruses are excellent pathogens for ………… specific narrow spectrum insecticidal applications.
Ans. Species

State True or False

1. Nosema locustae is a bacterium, which is used to control harmful grasshoppers.
Ans. False
2. Examples of aerobic nitrogen-fixing bacteria are Azomonas, Azospirillum.
Ans. True
3. Scientists have introduced genetic materials of Bacillus thuringiensis bacterium in corn and cotton plants.
Ans. True
4. Caudovirales is a fungus, which can control pests.
Ans. False
5. Except paddy field, cyanobacterial colony is formed in Cycas.
Ans. True
6. Viruses are also used as bio-control agents to kill insect pests.
Ans. True

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